> Lab Techniques and Other Information (... This is in construction! ...)


General Lab Rules and Lab Member Conduct

Lab Procedures and Protocols:

- Rat Behavioral Experiments

- Handling rats (movie1, movie2).
- Videos and tutorials for subcutaneous, intravenous, intramuscular and intraperitoneal injections. Article on Right vs Left IP injections.
- Von Frey touch sensitivity protocol. See Video.
- Tail Flick protocol. See Video.
- Elevated Plus Maze protocol. See Video.
- Light and Dark Box protocol. See Video.
- Open Field protocol

- Empathy Box protocol
- Memory Consolidation Experiments: Pretraining, Training.
- Traveling SalesRat Experiments: Pretraining and protocol.


- Recording Behavioral data with Lick and Run and ZTracker.
- Recording time stamped video data with VideoRecord and VideoAnalyze
- Using Labbook (electronic lab notebook)
- Analyzing data with the AnalyzeThis 1.3 Excel/Visual basic spreadsheet.
- Statistical analyses (SyStat). See also the online textbook Statsoft

- Tetrode Recordings

- Making tetrodes, building a hyperdrive, doing surgery, recovering a hyperdrive.
- Recordings with Neuralynx's Cheetah 5.
-
Tetrode turning with Labbook.

- Patch Clamp Recordings

- Solutions and slice preparation.
- Booting up/ Shuting down the rig.
- Patch clamp procedure: current clamp.
- Patch clamp procedure: reactive clamp.

- Recording data with Patch 8.0.
- Matlab functions to analyze the data

- Histology and Immunohistochemistry

- General procedures (obtaining the brain, storing the brain, slicing the brain)
- NISSL staining
- TOH staining

- Optogenetics

- Virus injections:surgery and protocol
- Acute recordings: preparing the dual electrode-optical fiber, surgery, rig components (light sources, data acquisition...)
- Using AcuteRecord2.0 to light-stimulate and record simultaneously.
- In vivo behaving recordings: Preparing and testing the wireless device

- Computational Modeling and Data Analyses

- General introduction to NEURON.
- General resources for Matlab.
- General resources for EMERGENT.
- General resources for LabView.
- Analyzing EEG and Spike data with the laboratory Matlab functions.

- Psyc 4/596L class: "Introduction to Neural Data Analyses"
- Psyc 4/503 class: "Introduction to Computational Neuroscience".
- Set of NEURON utilities and functions
.

- General Lab Procedures

- 3D printing: Ultimaker 2+ and SolidWorks.
- Backups (data, user files, shared files)
- General procedures (ordering, cleaning, card and key access, injuries-safety)