Method
We use young (20-30 days) Long-Evans rats from which 400mm thick slices are obtained. Slices are submerged in ACSF (mM: NACl, 124; NaH2CO3, 26; D-glucose, 10; KCl, 5; CaCl2, 2; MgSO4, 2; NaH2PO4, 1.2) at 31-32 oC and perfused at constant flow (2ml/min). Electrophysiological recordings in CA1 are achieved using extra-cellular recording microelectrodes (ACSF filled, 300-400 KW). All drugs are freshly prepared in ACSF and bath applied. Stimulation are administred through a unipolar glass electrode, filled with ACSF, and placed in the Stratum Radiatum. Stimulation and recording are monitored by oscilloscope and computer, and saved on disk for off-line analysis. Data analyses are performed by programs written in C, and using the Matlab software in a Windows95 environment.